I almost hate to ask, but how does this play into the wall opening you made for the tank?
If you have access to a table saw, you might be able to shave additional 2x4s to help shim the bottom.
The end to end difference you've found after leveling isn't a big deal. I feel like almost all of my larger tanks end up with some tiny level of difference. Front to back, 1/4" doesn't seem like a big deal, but starting to go over that raises my concern a bit. If you haven't yet, you should get some sheets of closed-cell foam to set the tank on. This will allow the tank to "self-level" a bit and help your numbers even more.
Leveling a tank is certainly important, but even more important is supporting the bottom (edges on a glass tank - entire bottom on an acrylic tank) evenly IMO. Torsion will much more easily compromise seams.